DIET-INDUCED OBESITY EFFECTS ON TESTICULAR HISTOPATHOLOGICAL CHANGES AND BECLIN-1 LEVEL IN A RAT MODEL

Document Type : Preliminary preprint short reports of original research

Authors

1 Department of Dermatology, Venereology, and Andrology Faculty of Medicine, Alexandria University.

2 Department of Pathology, Faculty of Medicine, Alexandria University.

3 Department Department of Medical Physiology, and Andrology, Faculty of Medicine, Alexandria University.

4 Department of Dermatology, Venereology & Andrology, Faculty of Medicine, Alexandria University

Abstract

Obesity is a complex pathophysiological process that negatively impacts a variety of tissue including the testes resulting in abnormality in spermatogenesis and in semen parameters.Autophagy plays a fundamental homeostatic role in inside the cell. Dysregulated autophagy was recognized with obesity. Melatonin is a well-established antioxidants treatment.

AIM OF THE WORK:
The aim of this study was to assess the effect of high fat diet on testicular histological tissues andthe level of Beclin-1, as a marker of autophagy, in a rat model.

PATIENTS AND METHODS:
Experimental animals: 30 adult male wistar rats, were kept under standard laboratory conditions and randomly divided into three groups. Group I (n=10): control group, Group II (n=10): high fat diet group and Group III (n=10): high fat diet group treated with melatonin.
Methods: After 8 weeks of induction of high fat diet, melatonin was given to the third group and the study ended at 16 week. Excision of testicular tissues was conducted.BECLIN1 level was determined using ELISA. Histological examination was performed.

Keywords

ALEXMED ePosters
Volume 6, Issue 1
January 2024
Pages 38-39

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