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Younis, L., Abdalla, D., Al Sherif, M., Aboraya, M. (2022). THE DIAGNOSTIC ROLE OF CHOLINE TRANSPORTER AND CALRETININ IMMUNOHISTOCHEMISTRY IN HIRSCHSPRUNG DISEASE: A COMPARATIVE STUDY. ALEXMED ePosters, 4(3), 16-17. doi: 10.21608/alexpo.2022.152328.1442
Layla Kamal Younis; Dina Abdalla; Mohammed Abd AlAziz Al Sherif; Mohamed Mohamed Aboraya. "THE DIAGNOSTIC ROLE OF CHOLINE TRANSPORTER AND CALRETININ IMMUNOHISTOCHEMISTRY IN HIRSCHSPRUNG DISEASE: A COMPARATIVE STUDY". ALEXMED ePosters, 4, 3, 2022, 16-17. doi: 10.21608/alexpo.2022.152328.1442
Younis, L., Abdalla, D., Al Sherif, M., Aboraya, M. (2022). 'THE DIAGNOSTIC ROLE OF CHOLINE TRANSPORTER AND CALRETININ IMMUNOHISTOCHEMISTRY IN HIRSCHSPRUNG DISEASE: A COMPARATIVE STUDY', ALEXMED ePosters, 4(3), pp. 16-17. doi: 10.21608/alexpo.2022.152328.1442
Younis, L., Abdalla, D., Al Sherif, M., Aboraya, M. THE DIAGNOSTIC ROLE OF CHOLINE TRANSPORTER AND CALRETININ IMMUNOHISTOCHEMISTRY IN HIRSCHSPRUNG DISEASE: A COMPARATIVE STUDY. ALEXMED ePosters, 2022; 4(3): 16-17. doi: 10.21608/alexpo.2022.152328.1442

THE DIAGNOSTIC ROLE OF CHOLINE TRANSPORTER AND CALRETININ IMMUNOHISTOCHEMISTRY IN HIRSCHSPRUNG DISEASE: A COMPARATIVE STUDY

Article 3, Volume 4, Issue 3, September 2022, Page 16-17  XML
Document Type: Preliminary preprint short reports of original research
DOI: 10.21608/alexpo.2022.152328.1442
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Authors
Layla Kamal Younis1; Dina Abdalla2; Mohammed Abd AlAziz Al Sherif3; Mohamed Mohamed Aboraya email 1
1Department of Pathology, Faculty of Medicine, Alexandria University.
2pathology department, faculty of medicine, Alexandria university, Egypt
3Department of Surgery, Armed Forces Medical Academy
Abstract
Hirschsprung’s disease is an ENS malformation, with absence of intrinsic gangliain the distal rectum and a variable length of proximal bowel. Microscopic picture in H&E stained slides include absence of ganglion cells in muscularispropria and submucosa, and hyperplasia of cholinergic nerves & neurites. Diagnosis is challenging as, it needs hard boring examination of numerous sections of the tiny suction biopsy which lake the deep submucosa & muscularispropria where the ganglion cells are abundant, searching for ganglion cells which has a normal sparse nature in the distal 1-2 cm of the rectum. So we need ancillary techniques to help in diagnosis and make it easier, previously it was used AChE EHC which needs fresh frozen sample & CR IHC. Here we study Choline Transporter IHC to be a surrogate for AChE EHC with the advantage of being used with FFPE tissue sections, and to help in cases with misleading calretinin results.
Keywords
HIRSCHSPRUNG; CALRETININ; CHOLINE TRANSPORTER
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