LIGHT MICROSCOPIC STUDY OF THE EFFECT OF PLATELET RICH PLASMA ON HEPATIC FIBROSIS IN ADULT MALE ALBINO RATS

Document Type : Preliminary preprint short reports of original research

Authors

Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University

Abstract

Liver fibrosis is one of the most common causes of mortality worldwide. Platelet rich plasma (PRP) is one of the newly emerging modalities that contributes to tissue-healing process.
Aim of the work:
To study the potential effect of platelet rich plasma on hepatic fibrosis induced by thioacetamide (TAA).
Material and Methods:
Material:
18 adult male albino rats were randomly divided into 3 groups:
Group I (control): received intraperitoneal (i.p) saline twice weekly for 7 weeks.
Group II (fibrosis): received i.p injection of TAA (200 mg/kg) twice weekly for 7 weeks.
Group III (treated): received i.p injection of TAA (200 mg/kg) twice weekly for 7 weeks and PRP starting from 4th week of TAA administration to the end of the experiment.
Methods:
• Liver fibrosis was induced by intraperitoneal injection of TAA dissolved in 0.9% normal saline in a dose of 200 mg⁄kg body weight twice a week for 7 weeks. Fifteen rats were used for the purpose of PRP preparation using double centrifugation technique.
• At the end of the experiment, all groups were sacrificed and liver was taken for light microscopic examination by H&E and trichrome stains.

Keywords

ALEXMED ePosters
Volume 5, Issue 1
January 2023
Pages 9-10

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